This study introduces a simple direct antioxidant assay, based on the reduction of the abts.+ radical cation, and compares it with the myoglobin/abts.+ assay.
17+ Abts Assay. Comparison of abts, dpph, frap, and orac assays for estimating. The video lecture specifies on abts assay.
Abts Antioxidant Capacity Assay Kit Abin3172702 from i1.abocdn.com
The assay described here involves the direct production of the blue/green. The abts assay is considered one of the most sensitive techniques to identify antioxidant activity, because the response of antioxidants involves faster reaction kinetics (chanput et al., 2016). Abts elisa peroxidase substrate is commonly used with peroxidases, but is amenable to many other enzymes.
This study introduces a simple direct antioxidant assay, based on the reduction of the abts.+ radical cation, and compares it with the myoglobin/abts.+ assay.
Abts is a trademark of roche. In the presence of active enzyme, the substrate becomes a soluble green. Aus wikipedia, der freien enzyklopädie. In the presence of antioxidants, the oxidized abts radical is reduced to abts in a concentration dependent manner.